In vitro potency test for evaluation of Clostridium perfringens type D epsilon toxoid
نویسندگان
چکیده
منابع مشابه
Evaluation of plant - produced Clostridium perfringens type D epsilon toxoid in a vaccine against enterotoxaemia in sheep
Enterotoxaemia (commonly known as pulpy kidney disease) is a disease of economic importance in sheep, with a mortality rate of between 10% and 30% in non-vaccinated lambs. Although it also affects calves and goats, the symptoms are less severe, causing a non-fatal subacute disease (Morris et al. 2012). The disease is precipitated when sheep are fed a rich ration, such as green pastures, or if t...
متن کاملEpsilon-toxin plasmids of Clostridium perfringens type D are conjugative.
Isolates of Clostridium perfringens type D produce the potent epsilon-toxin (a CDC/U.S. Department of Agriculture overlap class B select agent) and are responsible for several economically significant enterotoxemias of domestic livestock. It is well established that the epsilon-toxin structural gene, etx, occurs on large plasmids. We show here that at least two of these plasmids are conjugative...
متن کاملIn silico fusion of epsilon and beta toxin genes of Clostridium perfringens types D and B
Fusion protein technology represents the strategy to achieve rapid, efficient, and cost-effective proteinexpression. Epsilon and Beta toxins are the most potent Clostridial toxins and cause disease in animals.This study describes in silico fusion of Clostridium perfringens types D and B epsilon and beta toxin genesthat was used for cloning in E.coli. The etx and cpb genes were...
متن کاملFusion of Clostridium perfringens type D and B epsilon and beta toxin genes and it’s cloning in E. coli
Designing and producing a proper fusion construction is the most important problem of producing large quantities of a properly folded functional protein. This construction should have all necessary components of a real gene. A good designed fusion gene construction could be cloned into a good and suitable host. Clostridium perfringens is an important pathogen of humans and livestock and produce...
متن کاملStability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine [Estabilidade e toxicidade da protoxina epsilon de Clostridium perfringens tipo D]
Purified epsilon prototoxin of Clostridium perfringens type D was produced, purified, and detoxified by the stoiechiometric method of non-radioactive iodine incorporation. Different degrees of iodination were perfomed and the toxicity of the derivatives were analysed by in vivo studies. Toxicity decreased inversely to the iodine incorporation. Eletrophoretic analysis showed different levels of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Arquivos do Instituto Biológico
سال: 2013
ISSN: 1808-1657
DOI: 10.1590/s1808-16572013000400013